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KMID : 0364820220580020067
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Korean Journal of Microbiology
2022 Volume.58 No. 2 p.67 ~ p.74
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The gut microbiota of the globular sea urchin, Strongylocentrotus nudus, from its natural habitat
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Kim Min-Ji
Cha In-Tae
Park Soo-Je
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Abstract
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Marine invertebrates have been well-known a symbiotic relationship with marine microorganisms obtained during sea water filtering. Among the marine invertebrate species, the sea urchin grazes on seaweed. Although sea-urchin barren may occur, there is limited ecological knowledge. The gut bacterial population of six urchins collected from the Dokdo and Ulleungdo was studied in this work using the 16S rRNA gene amplicon technique and analyzed using operational taxonomic unit (OTU) method. Because of the high frequency of unclassified groups, these studies at the phylum and genus level were able to predict the presence of uncultivated bacterial species on the urchin gut. The genus Sulfurospirillum, which can use sulfur compounds and nitrogen oxides, was also abundant among Ulleungdo’s urchins. The urchin gut microbial communities were clustered with their habitat as a result of the beta-diversity (PCoA and UPGMA). Furthermore, the diversity indices for the urchin gut microbial community obtained using the OTU were comparable those of amplicon sequence variant method. Despite the fact that each study approach was subordinated to the clustering method and the reference database employed, the microbial community structures were similar. In conclusion, we detected microbial diversity and community structure in the gut of urchins in this study and found no significant differences between the OTU and ASV analysis methods.
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KEYWORD
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East sea, gut, microbiota, urchin
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